On October 15th, Nucleic Acid Research magazine published the latest research results of the novel long non-coding RNA CRGregulates Drosophila locomotor behavior in collaboration with Liu Li and Chen Runsheng of the Institute of Biophysics, Chinese Academy of Sciences. The study discovered the role of a long, noncoding RNA specific to the nervous system in regulating the behavior of Drosophila, and clarified the molecular mechanism of its regulation.
Long non-coding RNA is a type of non-coding RNA longer than 200nt. Although many long RNAs have been discovered, only a few long non-coding RNAs have been elucidated. In this study, a batch of long non-coding RNAs of Drosophila melanogaster was predicted by biological information. Through RT-PCR screening and in situ hybridization verification, one of the long non-coding RNAs, CASK Regulatory Gene (CRG), was found in the nervous system. With specific expression. Subsequently, through bioinformatics and experimental methods, the study determined that CRG is a long non-coding RNA. Through bioinformatics prediction, the conserved region of the DNA sequence encoding CRG was obtained, and the CRG-deficient strain was constructed accordingly. Compared with wild-type strains, CRG-deficient strains have defects in exercise ability. CRG salvage experiments further confirmed that CRG participates in the regulation of exercise ability.
Further, the study found that the coding gene CASK related to the motor behavior of Drosophila is located upstream of CRG. Compared with wild-type strains, the RNA and protein levels of CASK in CRG-deficient strains were partially reduced. The recovery experiment verified that CASK participates in CRG regulation of Drosophila motor behavior. Later, using the dual-fluorescence reporting system, it was found that CRG has a positive regulatory effect on the CASK promoter. Chromosome immunoprecipitation analysis showed that CRG may promote the binding of RNA polymerase II to the CASK promoter region. RNA immunoprecipitation results suggest that CRG interacts with RNA polymerase II. Mutation rescue experiments further determined the functional region of CRG involved in the recruitment of RNA polymerase II to the CASK promoter.
This study macroscopically revealed the function of a new long non-coding RNA to participate in the regulation of behavior, and deeply analyzed the mechanism of the function of the long non-coding RNA from the molecular level, further enriching the biological significance of the long non-coding RNA , Provides a new clue to reveal the pathogenesis of neurological disorders related to dyskinesia.
The research was supported by the Ministry of Science and Technology, the National Natural Science Foundation of China and the Chinese Academy of Sciences.
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