Human malondialdehyde (MDA) Elisa kit method

1. Separately set up blank wells (the blank control wells do not add samples and enzyme label reagents, the rest of the steps are the same), standard wells, sample wells to be tested. Add 50Î¼l of the standard product to the standard well of the enzyme-coated plate; add 40Î¼l of the sample diluent to the sample well of the enzyme-coated plate and then add 10Î¼l of the sample to be tested (the final dilution of the sample is 5 times) Add 50Î¼l of enzyme-labeled reagent to each well of human malondialdehyde (MDA), except for blank wells. Shake gently to mix, and incubate at 37 Â° C for 60 minutes.
2. Discard the liquid and spin dry. Fill each well with diluted washing liquid, shake for 30 seconds, shake off the washing liquid, and pat dry with absorbent paper. Repeat this 5 times, pat dry.
3. For each well of human malondialdehyde (MDA) Elisa kit, first add the developer A50Î¼l, then add the developer B50Î¼l, gently shake and mix, and develop the color for 15 minutes at 37 Â° C in the dark.
4. Remove the enzyme labeling plate and add 50Î¼l of stop solution to each well to stop the reaction (at this time, the blue will turn to yellow).
5. Determination of human malondialdehyde (MDA) Elisa kit: Zero the blank holes, and measure the absorbance value (OD value) of each well at 450nm wavelength. ? The measurement should be performed within 15 minutes after the addition of the stop solution.

6. Calculate the linear regression equation of the standard curve according to the concentration of the standard product and the corresponding OD value, and then calculate the corresponding sample concentration based on the OD value of the sample in the regression equation of human malondialdehyde (MDA). It can also be calculated using various application software. It should be remembered that since the sample is diluted, its actual concentration should be multiplied by the total dilution factor.

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Beijing Jiehui Biological sold the "Human Malondialdehyde (MDA) Elisa Kit" product to provide after-sales service to ensure product quality. Where you can buy our company's ELISA test kit, you can provide free testing services.

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