Indirect agglutination reaction (detection of rheumatoid factor)

principle

Rheumatoid factor (rheumatoid factor RF) is an antibody against human or animal IgG Fc segments, and is an autoantibody that uses denatured IgG as the target antigen. IgM type RF is considered to be the main type of RF and also the type determined by conventional methods in clinical immunoassays.

IgG is adsorbed on the polystyrene latex particles as a detection reagent. In the reaction medium, if the serum to be tested contains RF, it can react with the latex particles by agglutination. This is a common method for detecting IgM type RF, but this method can only be qualitative or semi-quantitative by titer, its sensitivity and specificity are not high, and can only detect IgM type RF in serum.

Detection method:

Latex particle agglutination test

That is, 1 ~ 2 drops of serum + Ig-latex particles to be tested at a certain dilution