What are the commonly used packing methods for gas chromatography columns? Why does a newly packed column need to be aged for a period of time? The following answers to the questions.
The quality of the stationary phase packing will directly affect the efficiency of the column. Usually, the pumping packing method is used, that is, one end of the chromatographic column is plugged with glass wool, connected to the vacuum pump, and the other end is connected to a funnel. The stationary phase is added under suction. Tap the column until the stationary phase no longer enters.
After installing, plug in glass wool. The packing requirements are to be filled evenly and tightly, avoiding gaps. After the packed chromatographic column is connected to the instrument, it should be tested for pressure and leak first, and then purged with a carrier gas at a constant temperature for several hours and then subjected to analysis. This is generally called the aging process of the column.
The purpose of aging is to remove the residual solvent, low boiling point impurities, low molecular weight fixing solution, etc. of the stationary phase, to make the baseline of the recorder straight, and to make the fixing solution have a redistribution process on the surface of the support body at the aging temperature, thus coating More uniform and firm.
After the chromatographic column is packed, after a period of aging, the column efficiency and performance are stable, so that it can be used.
Twenty-seven, what is the performance of the chromatographic column after failure? What is the reason for its failure? Answer: The failure of the column is mainly manifested by poor chromatographic separation and a significantly shorter retention time of the components.
The main reason for the failure of the chromatographic column is that for gas-solid chromatography, the activity or adsorption performance of the stationary phase is reduced, and for gas-liquid chromatography, it is caused by the gradual loss of stationary liquid during use.
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